---
description: A simple glimpse into the tool.
---

# Toy Example

Before reading this part make sure you have successfully completed the tool installation:

{% content-ref url="installation" %}
[installation](installation)
{% endcontent-ref %}

To get a sense of how the tool works we have assembled a small toy example, so you can understand how to operate the tool in a few quick steps.\
If all goes well, you should see results in **5 minutes**.

Download the following file and unzip it :

{% file src="https://2747853774-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F-LeG1a5HYCKC6GFmlh1i%2Fuploads%2FMiAJiAKmGdGg9WFBhFhO%2Ftoy_example.zip?alt=media&token=0546beb1-cc31-446f-9483-726a606fc210" %}

The zip contains 2 files :&#x20;

* design.csv
* toy\_data\_reads.fastq



Next, If the tool is not running then run it using :&#x20;

```
$ docker run -p 5000:5000 solqc/tool
```

Open your browser and go [here](http://localhost:5000/):

```
http://localhost:5000/
```

Once you are on this screen :&#x20;

<figure><img src="https://2747853774-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F-LeG1a5HYCKC6GFmlh1i%2Fuploads%2FsZTvxaSpR9gTa7ZIcTHL%2FScreenshot%202024-07-07%20at%200.55.14.png?alt=media&#x26;token=8528eff4-79fb-4511-8a14-9fc2e619b209" alt=""><figcaption></figcaption></figure>

Upload the design file (_design.csv_), using **Design**, then **Upload file**.

Now, upload the NGS file (_toy\_data\_reads.fastq)_ using **NGS Files,** then **Upload File**.\


Press on **Library Configuration.** You should see this:

<figure><img src="https://2747853774-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F-LeG1a5HYCKC6GFmlh1i%2Fuploads%2FZ8weeJKRDvJDCl5oPtgo%2FScreenshot%202024-07-07%20at%201.08.30.png?alt=media&#x26;token=61e251ac-01cf-4c7f-94b3-d64efebb4030" alt=""><figcaption></figcaption></figure>

Use the following configuration:

Prefix: _ACAACGCTTTCTGTGTCGTG_

Lengt&#x68;_: 210_

Barcode Start: _20_

Barcode End: _32_

Barcode Tolerance: _2_





Keep the default **Analysis Configurations** and press **Proceed to Overview**, you should see the following:

<figure><img src="https://2747853774-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F-LeG1a5HYCKC6GFmlh1i%2Fuploads%2FBPmtRk8DZKG2gB2DDxVq%2Fimage.png?alt=media&#x26;token=9fa7162c-5a37-40b4-866b-6ad6a054b9f2" alt=""><figcaption></figcaption></figure>

If all went well, after the analysis finishes you should see this:

<figure><img src="https://2747853774-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F-LeG1a5HYCKC6GFmlh1i%2Fuploads%2FxOwfNDSbmhKL2ieZGr1v%2FScreenshot%202024-07-07%20at%201.29.03.png?alt=media&#x26;token=4e453377-3453-49bc-9d73-d548c8f38a94" alt=""><figcaption></figcaption></figure>

This means the tool preformed an analysis on the given data and returned:&#x20;

1. The requested analysis report (in our case - _Error Rate per Base Table Analyzer_, _General Analyzer_).
2. A zip containing 2 csv files, one with the _Frequency Analyzer_ and one with the _Matching Analyzer_.



### Congratulations! You have just made your first SOLQC report!
