Toy Example
A simple glimpse into the tool.
Last updated
A simple glimpse into the tool.
Last updated
Before reading this part make sure you have successfully completed the tool installation:
To get a sense of how the tool works we have assembled a small toy example, so you can understand how to operate the tool in a few quick steps. If all goes well, you should see results in 5 minutes.
Download the following file and unzip it :
The zip contains 2 files :
design.csv
toy_data_reads.fastq
Next, If the tool is not running then run it using :
Open your browser and go here:
Once you are on this screen :
Upload the design file (design.csv), using Design, then Upload file.
Now, upload the NGS file (toy_data_reads.fastq) using NGS Files, then Upload File.
Press on Library Configuration. You should see this:
Use the following configuration:
Prefix: ACAACGCTTTCTGTGTCGTG
Length: 210
Barcode Start: 20
Barcode End: 32
Barcode Tolerance: 2
Keep the default Analysis Configurations and press Proceed to Overview, you should see the following:
If all went well, after the analysis finishes you should see this:
This means the tool preformed an analysis on the given data and returned:
The requested analysis report (in our case - Error Rate per Base Table Analyzer, General Analyzer).
A zip containing 2 csv files, one with the Frequency Analyzer and one with the Matching Analyzer.
