Toy Example

A simple glimpse into the tool.

Before reading this part make sure you have successfully completed the tool installation:

Installation

To get a sense of how the tool works we have assembled a small toy example, so you can understand how to operate the tool in a few quick steps. If all goes well, you should see results in 5 minutes.

Download the following file and unzip it :

882KB

toy_example.zip

archive

The zip contains 2 files :

• design.csv

• toy_data_reads.fastq

Next, If the tool is not running then run it using :

$ docker run -p 5000:5000 solqc/tool

Open your browser and go here:

http://localhost:5000/

Once you are on this screen :

Upload the design file (design.csv), using Design, then Upload file.

Now, upload the NGS file (toy_data_reads.fastq) using NGS Files, then Upload File.

Press on Library Configuration. You should see this:

Use the following configuration:

Prefix: ACAACGCTTTCTGTGTCGTG

Length: 210

Barcode Start: 20

Barcode End: 32

Barcode Tolerance: 2

Keep the default Analysis Configurations and press Proceed to Overview, you should see the following:

If all went well, after the analysis finishes you should see this:

This means the tool preformed an analysis on the given data and returned:

1. The requested analysis report (in our case - Error Rate per Base Table Analyzer, General Analyzer).

2. A zip containing 2 csv files, one with the Frequency Analyzer and one with the Matching Analyzer.

Congratulations! You have just made your first SOLQC report!